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Super Uber Clean Phyto Culturing!
Topic Started: Feb 25 2010, 05:31:06 PM (760 Views)
Post #61 Jun 29 2010, 06:18:13 AM Suzy
I think this is the way to go for me! I have it down to easy peasy now. I make up a bucket of water, fill as many 2l bottles that 5 gallons will make, then set them aside until I need them. Then, as I use a bottle, I add that same bubbler to the new bottle then inoculate it the next day. The next day, I just siphon from the oldest bottle and use the rest for the day. The densities are really strong and under a microscope, I see no contaminents!

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"i was informed of some dolphin related testing going on up there"
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Post #62 Jun 29 2010, 07:28:33 PM Amie
Very nice. So you are saying that it's only taking 4 days to go dark?
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stormy, stormy nights
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http://www.spawar.navy.mil/sandiego/technology/mammals/interns.html
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Post #63 Jun 30 2010, 05:52:32 AM Suzy
No, the darkest one here is 6 days. but, I am not sure if I wait longer, if I would be able to tell if it was denser or not? I would just be guessing, even under a microscope. When I put in the fafusa density ruler dodad, it disappears completely.
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Post #64 Jul 3 2010, 06:34:08 PM Amie
I would say that that is pretty dense. The only way to really tell if you are hitting the peak of its production before it declines is to use a hemocytometer and count the number of cells through the microscope. That way you can know the exact point when it starts to decline because the food source is depleted. But I think the '6-day' method is probably good enough.
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Post #65 Jul 3 2010, 06:34:32 PM Amie
We've got to find you a good microscope one of these days.
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Post #66 Jul 5 2010, 12:10:07 PM Suzy
Any suggestions?
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